Authors:
P Jung, C Sommer, FM Barriga, SJ Buczacki, X Hernando-Momblona, M Sevillano, M Duran-Frigola, P Aloy, M Selbach, DJ Winton, E Batlle
Journal name: 
Stem Cell Reports
Citation info: 
5(6):979-987
Abstract: 
Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to identify stem cell markers that would allow for purification of hCoSCs. In an unbiased approach, membrane-enriched protein fractions derived from in vitro human colonic organoids were analyzed by quantitative mass spectrometry. Protein tyrosine pseudokinase PTK7 specified a cell population within human colonic organoids characterized by highest self-renewal and re-seeding capacity. Antibodies recognizing the extracellular domain of PTK7 allowed us to isolate and expand hCoSCs directly from patient-derived mucosa samples. Human PTK7+ cells display features of canonical Lgr5+ ISCs and include a fraction of cells that undergo differentiation toward enteroendocrine lineage that resemble crypt label retaining cells (LRCs).
DOI: 
http://doi.org/10.1016/j.stemcr.2015.10.003
Research group: 
Winton Group
E-pub date: 
08 Dec 2015