Authors:
A Trinh, IH Rye, V Almendro, A Helland, HG Russnes, F Markowetz
Journal name: 
Genome Biol
Citation info: 
15(8):442
Abstract: 
Molecular analysis has revealed extensive intra-tumor heterogeneity in human cancer samples, but cannot identify cell-to-cell variations within the tissue microenvironment. In contrast, in situ analysis can identify genetic aberrations in phenotypically defined cell subpopulations while preserving tissue-context specificity. GoIFISHGoIFISH is a widely applicable, user-friendly system tailored for the objective and semi-automated visualization, detection and quantification of genomic alterations and protein expression obtained from fluorescence in situ analysis. In a sample set of HER2-positive breast cancers GoIFISHGoIFISH is highly robust in visual analysis and its accuracy compares favorably to other leading image analysis methods. GoIFISHGoIFISH is freely available at www.sourceforge.net/projects/goifish/.
DOI: 
http://doi.org/10.1186/s13059-014-0442-y
Research group: 
Markowetz Group
E-pub date: 
26 Aug 2014