Authors:
H Fernando, S Sewitz, J Darot, S Tavaré, JL Huppert, S Balasubramanian
Journal name: 
Nucleic Acids Res
Citation info: 
37(20):6716-6722
Abstract: 
G-quadruplex nucleic acids have been proposed to play a role in a number of fundamental biological processes that include transcription and translation. We have developed a single-chain antibody that is selective for G-quadruplex DNA over double-stranded DNA, and here show that when it is expressed in human cells, it significantly affects the expression of a wide variety of genes, in a manner that correlates with the presence of predicted G-quadruplexes. We observe cases where gene expression is increased or decreased, and that there are apparent interactions with G-quadruplex motifs at the beginning and end of the genes, and on either strand. The outcomes of this genome-wide study demonstrate that G-quadruplex recognition by the antibody has physiological consequences, and provides insights into some of the complexity associated with G-quadruplex-based regulation.
DOI: 
http://doi.org/10.1093/nar/gkp740
Research group: 
Balasubramanian Group, Tavaré Group
E-pub date: 
01 Nov 2009