Authors:
D Schmidt, R Stark, MD Wilson, GD Brown, DT Odom
Journal name: 
PLoS One
Citation info: 
3(11):e3713
Abstract: 
Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers. Here we report a protocol, Mab-seq, that generates genome-scale quality evaluations for nucleic acid libraries intended for deep-sequencing. We show how commercially available genomic microarrays can be used to maximize the efficiency of library creation and quickly generate reliable preliminary data on a chromosomal scale in advance of deep sequencing. We also exploit this technique to compare enriched regions identified using microarrays with those identified by sequencing, demonstrating that they agree on a core set of clearly identified enriched regions, while characterizing the additional enriched regions identifiable using HTP sequencing.
DOI: 
http://doi.org/10.1371/journal.pone.0003713
Research group: 
Odom Group
E-pub date: 
31 Aug 2008