Authors:
A Bric, C Miething, CU Bialucha, C Scuoppo, L Zender, A Krasnitz, Z Xuan, J Zuber, M Wigler, J Hicks, RW McCombie, MT Hemann, GJ Hannon, S Powers, SW Lowe
Journal name: 
Cancer Cell
Citation info: 
16(4):324-335
Abstract: 
Short hairpin RNAs (shRNAs) capable of stably suppressing gene function by RNA interference (RNAi) can mimic tumor-suppressor-gene loss in mice. By selecting for shRNAs capable of accelerating lymphomagenesis in a well-characterized mouse lymphoma model, we identified over ten candidate tumor suppressors, including Sfrp1, Numb, Mek1, and Angiopoietin 2. Several components of the DNA damage response machinery were also identified, including Rad17, which acts as a haploinsufficient tumor suppressor that responds to oncogenic stress and whose loss is associated with poor prognosis in human patients. Our results emphasize the utility of in vivo RNAi screens, identify and validate a diverse set of tumor suppressors, and have therapeutic implications.
DOI: 
http://doi.org/10.1016/j.ccr.2009.08.015
Research group: 
Hannon Group
E-pub date: 
06 Oct 2009