Authors:
DC Rio, M Ares, GJ Hannon, TW Nilsen
Journal name: 
Cold Spring Harb Protoc
Citation info: 
2010(6):pdb.prot5440
Abstract: 
Recovery of deproteinized RNA almost always is performed via precipitation with ethanol. If the RNA is free of contaminants (i.e., it is well extracted) as determined by spectrophotometry, it will resuspend easily and be ready for any subsequent manipulation. Ethanol precipitation is quite useful because it provides quantitative recovery of any-sized RNA, from several kilobases to 20 nucleotides (nt). Major considerations in this protocol are RNA concentration, pellet identification, and resuspension.
DOI: 
http://doi.org/10.1101/pdb.prot5440
Research group: 
Hannon Group
E-pub date: 
01 Jun 2010
Users with this publication listed: 
Greg Hannon