S Bornemann, S Balasubramanian, JR Coggins, C Abell, DJ Lowe, RN Thorneley
Journal name: 
Biochem J
Citation info: 
305 ( Pt 3):707-710
We report the observation of a deuterium kinetic isotope effect for the conversion of 5-enolpyruvylshikimate-3-phosphate into chorismate (6proR2HV = 1.13 +/- 0.03) using recombinant chorismate synthase from Escherichia coli. Similar isotope effects were observed for the decay of a spectroscopically characterized flavin intermediate (6proR2Hk = 1.17 +/- 0.04) during single-turnover experiments. The main rate-limiting steps and C-(6proR)-H bond breaking are therefore distinct and both must occur after the formation of the flavin intermediate and either before or concomitant with its decay.
Research group: 
Balasubramanian Group
E-pub date: 
31 Jan 1995
Users with this publication listed: 
Shankar Balasubramanian