Authors:
KM Braun, FM Watt
Journal name: 
J Investig Dermatol Symp Proc
Citation info: 
9(3):196-201
Abstract: 
Epidermal label-retaining cells (LRC) can be identified by giving neonatal mice repeated injections of 3H-thymidine or 5-bromo-2'-deoxyuridine and then finding the cells that are still labelled in adulthood. Although label retention is simply a marker of the proliferative history of a cell, there is, nevertheless, evidence that it is a characteristic of epidermal stem cells. Here we review the early literature on LRC and then highlight two recent applications. We describe how LRC can be visualized by whole-mount labelling of the epidermis and how purified LRC can be used to screen for markers of the epidermal stem cell compartment.
DOI: 
http://doi.org/10.1111/j.1087-0024.2004.09313.x
Research group: 
Watt Group
E-pub date: 
01 Sep 2004