Authors:
G Biffi, D Tannahill, J Miller, WJ Howat, S Balasubramanian
Journal name: 
PLoS One
Citation info: 
9(7):e102711
Abstract: 
Four-stranded G-quadruplex DNA secondary structures have recently been visualized in the nuclei of human cultured cells. Here, we show that BG4, a G-quadruplex-specific antibody, can be used to stain DNA G-quadruplex structures in patient-derived tissues using immunohistochemistry. We observe a significantly elevated number of G-quadruplex-positive nuclei in human cancers of the liver and stomach as compared to background non-neoplastic tissue. Our results suggest that G-quadruplex formation can be detected and measured in patient-derived material and that elevated G-quadruplex formation may be a characteristic of some cancers.
DOI: 
http://doi.org/10.1371/journal.pone.0102711
Research group: 
Balasubramanian Group
E-pub date: 
31 Aug 2014