Authors:
SA Rahman, SM Cuesta, N Furnham, GL Holliday, JM Thornton
Journal name: 
Nat Methods
Citation info: 
11(2):171-174
Abstract: 
We present EC-BLAST (http://www.ebi.ac.uk/thornton-srv/software/rbl/), an algorithm and Web tool for quantitative similarity searches between enzyme reactions at three levels: bond change, reaction center and reaction structure similarity. It uses bond changes and reaction patterns for all known biochemical reactions derived from atom-atom mapping across each reaction. EC-BLAST has the potential to improve enzyme classification, identify previously uncharacterized or new biochemical transformations, improve the assignment of enzyme function to sequences, and assist in enzyme engineering.
DOI: 
http://doi.org/10.1038/nmeth.2803
E-pub date: 
01 Feb 2014