Authors:
SV Lensing, G Marsico, R Hänsel-Hertsch, EY Lam, D Tannahill, S Balasubramanian
Journal name: 
Nat Methods
Citation info: 
13(10):855-857
Abstract: 
Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.
DOI: 
http://doi.org/10.1038/nmeth.3960
Research group: 
Balasubramanian Group
E-pub date: 
31 Oct 2016