Authors:
AA Serandour, GD Brown, JD Cohen, JS Carroll
Journal name: 
Genome Biol
Citation info: 
14(12):R147
Abstract: 
ChIP-exonuclease (ChIP-exo) is a modified ChIP-seq approach for high resolution mapping of transcription factor DNA sites. We describe an Illumina-based ChIP-exo method which provides a global improvement of the data quality of estrogen receptor (ER) ChIP and insights into the motif structure for key ER-associated factors. ChIP-exo of the ER pioneer factor FoxA1 identifies protected DNA with a predictable 8 bp overhang from the Forkhead motif, which we term mesas. We show that mesas occur in multiple cellular contexts and exist as single or overlapping motifs. Our Illumina-based ChIP-exo provides high resolution mapping of transcription factor binding sites.
DOI: 
http://doi.org/10.1186/gb-2013-14-12-r147
Research group: 
Carroll Group
E-pub date: 
30 Nov 2013
Users with this publication listed: 
Jason Carroll