H Stöckmann, AA Neves, S Stairs, H Ireland-Zecchini, KM Brindle, FJ Leeper
Journal name: 
Chem Sci
Citation info: 
Two reagents have been synthesized for selective labeling of cell surface azidoglycans, an unusually stable version of a dibenzocyclooctyne (TMDIBO) and a third-generation difluorinated cyclooctyne (DIFO3). Both syntheses are efficient with minimal purification, and the dibenzocyclooctyne is stable under basic and acidic conditions. Flow cytometric measurements with azidosugar labeled cancer cells, in which these reagents were linked to the fluorophore Alexa Fluor 647, gave a signal-to-background ratio of up to 35 with TMDIBO as compared to ≈10 for DIFO3 and ≈5 for a phosphine reagent. TMDIBO-based probes should have applications in molecular imaging of cell surface glycans in vivo.
Research group: 
Brindle Group
E-pub date: 
31 May 2011
Users with this publication listed: 
Kevin Brindle