Authors:
KA Holmes, GD Brown, JS Carroll
Journal name: 
Methods Mol Biol
Citation info: 
1366:79-98
Abstract: 
Chromatin immunoprecipitation-sequencing (ChIP-Seq) is a powerful tool which combines the established method of ChIP with next-generation sequencing (NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.
DOI: 
http://doi.org/10.1007/978-1-4939-3127-9_8
Research group: 
Carroll Group
E-pub date: 
31 Aug 2016