Authors:
P Jeggle, ESJ Smith, AP Stewart, S Haerteis, C Korbmacher, JM Edwardson
Journal name: 
Biochem Biophys Res Commun
Citation info: 
464(1):38-44
Abstract: 
ASIC and ENaC are co-expressed in various cell types, and there is evidence for a close association between them. Here, we used atomic force microscopy (AFM) to determine whether ASIC1a and ENaC subunits are able to form cross-clade hybrid ion channels. ASIC1a and ENaC could be co-isolated from detergent extracts of tsA 201 cells co-expressing the two subunits. Isolated proteins were incubated with antibodies against ENaC and Fab fragments against ASIC1a. AFM imaging revealed proteins that were decorated by both an antibody and a Fab fragment with an angle of ∼120° between them, indicating the formation of ASIC1a/ENaC heterotrimers.
DOI: 
http://doi.org/10.1016/j.bbrc.2015.05.091
E-pub date: 
14 Aug 2015