G Biffi, D Tannahill, S Balasubramanian
Journal name: 
J Am Chem Soc
Citation info: 
Telomeric repeat-containing RNA (TERRA) is important for telomere regulation, but the structural basis for how TERRA localizes to chromosome ends is unknown. Here we report on studies exploring whether the TERRA G-quadruplex structure is critical for binding to telomeres. We demonstrate that the telomeric protein TRF2 binds TERRA via interactions that necessitate the formation of a G-quadruplex structure rather than the TERRA sequence per se. We also show that TRF2 simultaneously binds TERRA and telomeric duplex or G-quadruplex DNA. These observations suggest that the TERRA G-quadruplex is a key feature of telomere organization.
Research group: 
Balasubramanian Group
E-pub date: 
25 Jul 2012
Users with this publication listed: 
David Tannahill
Shankar Balasubramanian