Authors:
S Aldridge, S Watt, MA Quail, T Rayner, M Lukk, MF Bimson, D Gaffney, DT Odom
Journal name: 
Genome Biol
Citation info: 
14(11):R124
Abstract: 
ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days.
DOI: 
http://doi.org/10.1186/gb-2013-14-11-r124
Research group: 
Odom Group
E-pub date: 
07 Nov 2013