Authors:
CP Martinez-Jimenez, N Eling, H-C Chen, CA Vallejos, AA Kolodziejczyk, F Connor, L Stojic, TF Rayner, MJT Stubbington, SA Teichmann, M de la Roche, JC Marioni, DT Odom
Journal name: 
Science
Citation info: 
355(6332):1433-1436
Abstract: 
Aging is characterized by progressive loss of physiological and cellular functions, but the molecular basis of this decline remains unclear. We explored how aging affects transcriptional dynamics using single-cell RNA sequencing of unstimulated and stimulated naïve and effector memory CD4+ T cells from young and old mice from two divergent species. In young animals, immunological activation drives a conserved transcriptomic switch, resulting in tightly controlled gene expression characterized by a strong up-regulation of a core activation program, coupled with a decrease in cell-to-cell variability. Aging perturbed the activation of this core program and increased expression heterogeneity across populations of cells in both species. These discoveries suggest that increased cell-to-cell transcriptional variability will be a hallmark feature of aging across most, if not all, mammalian tissues.
DOI: 
http://doi.org/10.1126/science.aah4115
Research group: 
Odom Group, Marioni Group, de la Roche Group
E-pub date: 
31 Mar 2017