Authors:
RJ Simpson, KM Brindle, FF Brown, ID Campbell, DL Foxall
Journal name: 
Biochem J
Citation info: 
202(3):573-579
Abstract: 
A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of n.m.r. to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by n.m.r. are considered.
DOI: 
http://doi.org/10.1042/bj2020573
Research group: 
Brindle Group
E-pub date: 
28 Feb 1982
Users with this publication listed: 
Kevin Brindle