Authors:
N Erard, SRV Knott, GJ Hannon
Journal name: 
Mol Cell
Citation info: 
67(2):348-354.e4
Abstract: 
We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.
DOI: 
http://doi.org/10.1016/j.molcel.2017.06.030
Research group: 
Hannon Group
E-pub date: 
20 Jul 2017