Authors:
N Nelson, M Lopez-Pelaez, A Palazon, E Poon, M De La Roche, S Barry, V Valge-Archer, RW Wilkinson, SJ Dovedi, PD Smith
Journal name: 
Oncoimmunology
Citation info: 
8(8):1599635
Abstract: 
In vitro assays that evaluate CD8+ T cell-mediated cytotoxicity are important to aid in the development of novel therapeutic approaches to enhance anti-tumor immune responses. Here, we describe a novel cytotoxicity co-culture assay that circumvents the problem of highly variable allogeneic responses and obviates the constraints of HLA-restriction between effector and target cells. We show that this assay can be easily applied to a panel of tumor cell lines to provide additional insights into intrinsic drivers of sensitivity/resistance to T cell-mediated killing, and to evaluate the impact of targeted therapies on both tumor and T cell compartments.
DOI: 
http://doi.org/10.1080/2162402X.2019.1599635
Research group: 
de la Roche Group
E-pub date: 
01 Jan 2019
Users with this publication listed: 
Maike de la Roche