Authors:
F Mitsumori, D Rees, KM Brindle, GK Radda, ID Campbell
Journal name: 
Biochim Biophys Acta
Citation info: 
969(2):185-193
Abstract: 
31P-NMR measurements of saturation transfer have been used to measure the flux between Pi and ATP in Escherichia coli cells respiring on an endogenous carbon source. Measurements were made in the wild type and in cells genetically modified to give a 5-fold higher concentration of the F1F0-ATP synthase. The flux in the two cell types was not significantly different. This, together with studies using inhibitors specific for the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase and the ATP synthase, suggests that the observed flux arises predominantly from glycolytic rather than ATP synthase activity. Although this conclusion is in disagreement with previous experiments on E. coli, it is in agreement with recent experiments on yeast.
DOI: 
http://doi.org/10.1016/0167-4889(88)90074-2
Research group: 
Brindle Group
E-pub date: 
31 Mar 1988
Users with this publication listed: 
Kevin Brindle