Authors:
SM Huber, P van Delft, A Tanpure, EA Miska, S Balasubramanian
Journal name: 
J Am Chem Soc
Citation info: 
139(5):1766-1769
Abstract: 
5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.
DOI: 
http://doi.org/10.1021/jacs.6b12180
Research group: 
Balasubramanian Group
E-pub date: 
08 Feb 2017