Authors:
FA Gallagher, MI Kettunen, SE Day, M Lerche, KM Brindle
Journal name: 
Magn Reson Med
Citation info: 
60(2):253-257
Abstract: 
Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of (13)C MR spectroscopy (MRS). [5-(13)C(1)]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000-fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using (13)C-MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation.
DOI: 
http://doi.org/10.1002/mrm.21650
Research group: 
Brindle Group
E-pub date: 
31 Aug 2008