6th– 7th May 2020 at Cancer Research UK Cambridge Institute

Welcome to the third ‘Applications of in situ hybridisation in research and disease’ symposium.

The Symposium is intended to provide an overview of the current accepted methods for different in situhybridisation applications and for networking with experts for those new to the field. The meeting will highlight advances in in situ hybridisation and its application in disease. We hope to bring together researchers across any field interested in in situ hybridisation. To achieve these goals are assembling an outstanding list of world-leading researchers to present their work.

Sessions will include Currently accepted methods, Emerging techniques, and translational research. Each session will have 5 talks as well as a panel discussion where the audience can ask questions to the session speakers. There are also lots of opportunities for networking and problem solving.

There are opportunities for delegates to present their work in short talks and posters – more details to follow.

Scientific Organising Committee

Prof Scott Fraser, University Southern California, USA

Scott E. Fraser, Ph.D., has a long-standing commitment to quantitative biology, applying the tools of chemistry, engineering and physics to problems in biology and medicine. His personal research centers on imaging and molecular analysis of intact biological systems, with an emphasis on early development, organogenesis and medical diagnostics.

After training in physics (B.S., Harvey Mudd College, 1976) and biophysics (Ph.D., Johns Hopkins University, 1979), he joined the faculty at UC Irvine and rose through the ranks to become Chair of the Department of Physiology and Biophysics. In 1990 he moved to Caltech to serve as the Anna L. Rosen Professor of Biology and the Director of the Biological Imaging Center. He is deeply committed to interdisciplinary training and translational research, having helped found the Caltech Brain Imaging Center and the Kavli Institute of Nanoscience, as well as serving as the Director of the Rosen Center for Biological Engineering.

In fall of 2012, he moved to USC to take a Provost Professorship in the Dornsife College of Letters, Arts and Sciences, Children’s Hospital Los Angeles, Keck School of Medicine and the Viterbi School of Engineering. He remains active in interdisciplinary research and serves as the Director of Science Initiatives as well as the Elizabeth Garrett Chair of Convergent Biosciences for the USC campuses.

Dr. Fraser is a Fellow of the European Academy of Science, the AAAS and the American Academy of Arts and Science and is also a Fellow to the American Institute for Medical and Biological Engineering College as well as the National Academy of Inventors.

Prof Jay Lee, Cold Spring Harbor Labs, USA

Prof Lee received his B.A. degree from the University of Pennsylvania. He received M.D. Ph.D from Tufts University, where he studied the implication of cell cycle and transcriptional heterogeneity in tissue patterning. He then entered internal medicine residency at the University of Pennsylvania, until he joined the laboratory of George Church at Harvard Medical School and Wyss Institute of Bio-inspired Engineering, Boston in 2006. As a post-doctoral fellow, he collaborated on NGS technology and application development, engineered human iPS-cells for functional genomics, and developed in-situ RNA sequencing methods. He joined Cold Spring Harbor Lab as a faculty in 2014, and his lab is investigating cell-cell interactions and mechanical forces that modulate morphogen and growth factor signaling in normal development and in cancer, as well as developing technologies for reconstructing spatiotemporally resolved gene expression at single cell resolution in situ.

Dr Julia Jones, University of Cambridge, UK

Dr Jones did a BSc (Hons) Biomedical Science at University of Southampton and a PhD in Neuroscience at University of Cambridge (Babraham Institute) and has experience in the Pharmaceutical Industry at Eli Lilly and GlaxoSmithKline. Julia joined the Cancer Research UK Cambridge Institute (CI) Histopathology/ISH core facility at the beginning of 2007 and is the Scientific Manager for ISH. The ISH Service is offered for researchers (and collaborators) based at the CI. Current methods include automated RNAscope and BaseScope for detection of mRNA and ncRNA, Locked Nucleic Acid ISH for detection of miRNA, and FISH for detecting DNA.

Dr Romualdo Ciau-Uitz, University of Oxford, UK

Aldo Ciau-Uitz is a developmental and stem cell biologist at the MRC Molecular Haematology Unit, The Weatherall Institute of Molecular Medicine, University of Oxford. Aldo received his Ph.D. degree in Molecular Biology (Developmental Biology) from King’s College London in 2001 and, after undertaking a postdoctoral position in the University of Nottingham, he joined the MRC Molecular Haematology Unit in Oxford. Aldo is interested in the development of the haematopoietic system, in particular how haematopoietic stem cells are created during embryogenesis. He established Xenopus as a unique model for the study of ontogeny of this tissue stem cells. Aldo has extensively used chromogenic in situ hybridisation on whole embryos as well as tissue sections to establish the step wise programming of haematopoietic stem cells and, as a result, he has deposited thousands of in situ hybridisation images into Xenbase, the Xenopus model organism database. Aldo is currently interested in translating his findings in embryos to the establishment of protocols for the in vitro differentiation of haematopoietic stem cells from pluripotent cells.

Dr Will Howat, Abcam, UK

Dr Paul Murdock, Asterand, UK

Dr Boye Schnack-Nielsen, Bioneer, DK